CCL2 ELISA Kit for Human(Homo sapiens) | EIAab.comCCL2 ELISA Kit for Human(Homo sapiens)

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Index > Protein center > CCL2(Gene name) > Human

CCL2 (Gene name),

C-C motif chemokine 2 (Protein name ), CCL2_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

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Gene name:

CCL2(MCP1;SCYA2);

Protein name:

C-C motif chemokine 2;

Alternative:

Monocyte chemoattractant protein 1;HC11;Monocyte chemotactic protein 1(MCP-1);Monocyte chemotactic and activating factor(MCAF);Small-inducible cytokine A2;Monocyte secretory protein JE;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Monomer or homodimer; in equilibrium. Binds to CCR2 and CCR4. Is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans.

Function:

Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.

Subcellular Location:

Secreted

Protein Attributes:

Sequence length:

Sequence:

50:

99:

YRRITSSKCP | KEAVIFKTIV | AKEICADPKQ | KWVQDSMDHL | DKQTQTPKT

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

Pfam:

PF00048

UniGene:

Hs.303649

MIM:

158105

SMR:

P13500

KEGG:

hsa:6347

String:

P13500

STRING:

9606.ENSP00000225831

Uniprot:

P13500

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ELISA Kit for Human C-C motif chemokine 2

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ELISA Kit for Human C-C motif chemokine 2

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E0087h

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96T

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ELISA Kit for Human C-C motif chemokine 2

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E0087m

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96T

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ELISA Kit for Human C-C motif chemokine 2

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E0087b

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ELISA Kit for Human C-C motif chemokine 2

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E0087r

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96T

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ELISA Kit for Human C-C motif chemokine 2

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E0087p

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96T

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ELISA Kit for Human C-C motif chemokine 2

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E0087d

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96T

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CLIA Kit for Human C-C motif chemokine 2

Cat.:

U0087p

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96T

CLIA Kit for Human C-C motif chemokine 2

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U0087r

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96T

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CLIA Kit for Human C-C motif chemokine 2

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U0087d

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96T

CLIA Kit for Human C-C motif chemokine 2

Cat.:

U0087b

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96T

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CLIA Kit for Human C-C motif chemokine 2

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U0087m

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Looking for manual.

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96T

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CLIA Kit for Human C-C motif chemokine 2

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U0087h

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CLIA Kit for Human C-C motif chemokine 2

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Polyclonal Antibody for Human C-C motif chemokine 2

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P0087Rb-r

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40ug/0.2ml

Polyclonal Antibody for Human C-C motif chemokine 2

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P0087Rb-m

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Polyclonal Antibody for Human C-C motif chemokine 2

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P0087Rb-h

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40ug/0.2ml

Monoclonal Antibody for Human C-C motif chemokine 2

Protein for Human C-C motif chemokine 2

Cat.:

R0087b

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1mg

Purity:

>90%

Protein for Human C-C motif chemokine 2

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R0087d

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1mg

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>90%

Protein for Human C-C motif chemokine 2

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R0087p

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1mg

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>90%

Protein for Human C-C motif chemokine 2

Cat.:

R0087h

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1mg

Purity:

>90%

Protein for Human C-C motif chemokine 2

R&D Technical Data

12% SDS-PAGE analysis

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of CCL2 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	91-99
Plasma	96	94-103

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CCL2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-98%	96-103%	97-105%	102-108%
EDTA plasma(n=5)	96-101%	98-105%	99-106%	91-96%
heparin plasma(n=5)	90-103%	91-104%	94-105%	95-102%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Cloning and sequencing of the cDNA for human monocyte chemotactic and activating factor (MCAF)."
Furutani Y. , Nomura H. , Notake M. , Oyamada Y. , Fukui T. , Yamada M. , Larsen C.G. , Oppenheim J.J. , Matsushima K.
Biochem. Biophys. Res. Commun.159:249-255(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
2.

"The human homolog of the JE gene encodes a monocyte secretory protein."
Rollins B.J. , Stier P. , Ernst T. , Wong G.G.
Mol. Cell. Biol.9:4687-4695(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]
3.

"Human monocyte chemoattractant protein-1 (MCP-1). Full-length cDNA cloning, expression in mitogen-stimulated blood mononuclear leukocytes, and sequence similarity to mouse competence gene JE."
Yoshimura T. , Yuhki N. , Moore S.K. , Appella E. , Lerman M.I. , Leonard E.J.
FEBS Lett.244:487-493(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
tissue: Glial tumor.
4.

"Cloning and expression of a gamma-interferon-inducible gene in monocytes: a new member of a cytokine gene family."
Chang H.C. , Hsu F. , Freeman G.J. , Griffin J.D. , Reinherz E.L.
Int. Immunol.1:388-397(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
5.

"Structure of human monocyte chemotactic protein gene and its regulation by TPA."
Shyy Y.J. , Li Y.S. , Kolattukudy P.E.
Biochem. Biophys. Res. Commun.169:346-351(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
6.

"Human monocyte chemoattractant protein-1 (MCP-1)."
Yoshimura T. , Leonard E.J.
Adv. Exp. Med. Biol.305:47-56(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
7.

"The expression of monocyte chemotactic protein (MCP-1) in human vascular endothelium in vitro and in vivo."
Li Y.S. , Shyy Y.J. , Wright J.G. , Valente A.J. , Cornhill J.F. , Kolattukudy P.E.
Mol. Cell. Biochem.126:61-68(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
8.

"Differential transcriptional regulation of the monocyte-chemoattractant protein-1 (MCP-1) gene in tumorigenic and non-tumorigenic HPV 18 positive cells: the role of the chromatin structure and AP-1 composition."
Finzer P. , Soto U. , Delius H. , Patzelt A. , Poustka A. , Coy J.F. , zur Hausen H. , Roesl F.
Oncogene19:3235-3244(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
9.

"Protein with cytokine activity, recombinant DNA, expression vector and hosts for obtaining it."
Caput D. , Ferrara P. , Miloux B. , Minty A. , Vita N.

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
10.

"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N. , Chen X. , Rolfs A. , Halleck A. , Hines L. , Eisenstein S. , Koundinya M. , Raphael J. , Moreira D. , Kelley T. , LaBaer J. , Lin Y. , Phelan M. , Farmer A.
Submitted (2003-05) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
11.

SeattleSNPs variation discovery resource
Submitted (2002-06) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
12.

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Urinary bladder.
13.

Mural R.J. , Istrail S. , Sutton G.G. , Florea L. , Halpern A.L. , Mobarry C.M. , Lippert R. , Walenz B. , Shatkay H. , Dew I. , Miller J.R. , Flanigan M.J. , Edwards N.J. , Bolanos R. , Fasulo D. , Halldorsson B.V. , Hannenhalli S. , Turner R. , more...
Submitted (2005-09) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
14.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Pancreas.
15.

"Complete amino acid sequence of a human monocyte chemoattractant, a putative mediator of cellular immune reactions."
Robinson E.A. , Yoshimura T. , Leonard E.J. , Tanaka S. , Griffin P.R. , Shabanowitz J. , Hunt D.F. , Appella E.
Proc. Natl. Acad. Sci. U.S.A.86:1850-1854(1989) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 24-99;PYROGLUTAMATE FORMATION AT GLN-24
16.

"Identification of the monocyte chemotactic protein from human osteosarcoma cells and monocytes: detection of a novel N-terminally processed form."
Decock B. , Conings R. , Lenaerts J.-P. , Biliau A. , van Damme J.
Biochem. Biophys. Res. Commun.167:904-909(1990) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 29-53 AND 82-92
17.

"Assignment of the human small inducible cytokine A2 gene, SCYA2 (encoding JE or MCP-1), to 17q11.2-12: evolutionary relatedness of cytokines clustered at the same locus."
Rollins B.J. , Morton C.C. , Ledbetter D.H. , Eddy R.L. Jr. , Shows T.B.
Genomics10:489-492(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GENE STRUCTURE
18.

"Structure/activity analysis of human monocyte chemoattractant protein-1 (MCP-1) by mutagenesis. Identification of a mutated protein that inhibits MCP-1-mediated monocyte chemotaxis."
Zhang Y.J. , Rutledge B.J. , Rollins B.J.
J. Biol. Chem.269:15918-15924(1994) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: MUTAGENESIS
19.

"Deletion of the NH2-terminal residue converts monocyte chemotactic protein 1 from an activator of basophil mediator release to an eosinophil chemoattractant."
Weber M. , Uguccioni M. , Baggiolini M. , Clark-Lewis I. , Dahinden C.A.
J. Exp. Med.183:681-685(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: EFFECT OF DELETION OF N-TERMINAL RESIDUES
20.

"Structural characterization of a monomeric chemokine: monocyte chemoattractant protein-3."
Kim K.-S. , Rajarathnam K. , Clark-Lewis I. , Sykes B.D.
FEBS Lett.395:277-282(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBUNIT
21.

"Lysine 58 and histidine 66 at the C-terminal alpha-helix of monocyte chemoattractant protein-1 are essential for glycosaminoglycan binding."
Chakravarty L. , Rogers L. , Quach T. , Breckenridge S. , Kolattukudy P.E.
J. Biol. Chem.273:29641-29647(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GAG BINDING SITES LYS-81 AND HIS-89;MUTAGENESIS OF LYS-81; HIS-89 AND 95-GLN--THR-99
22.

"Monomeric monocyte chemoattractant protein-1 (MCP-1) binds and activates the MCP-1 receptor CCR2B."
Paavola C.D. , Hemmerich S. , Grunberger D. , Polsky I. , Bloom A. , Freedman R. , Mulkins M. , Bhakta S. , McCarley D. , Wiesent L. , Wong B. , Jarnagin K. , Handel T.M.
J. Biol. Chem.273:33157-33165(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: HOMODIMERIZATION;MUTAGENESIS OF PRO-31; VAL-32; THR-33 AND TYR-36
23.

"Identification of surface residues of the monocyte chemotactic protein 1 that affect signaling through the receptor CCR2."
Jarnagin K. , Grunberger D. , Mulkins M. , Wong B. , Hemmerich S. , Paavola C. , Bloom A. , Bhakta S. , Diehl F. , Freedman R. , McCarley D. , Polsky I. , Ping-Tsou A. , Kosaka A. , Handel T.M.
Biochemistry38:16167-16177(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: MUTAGENESIS OF 25-PRO--PRO-31; ASP-26; ILE-28; ASN-29; PRO-31; VAL-32 AND THR-33
24.

"Identification of residues in the monocyte chemotactic protein-1 that contact the MCP-1 receptor, CCR2."
Hemmerich S. , Paavola C. , Bloom A. , Bhakta S. , Freedman R. , Grunberger D. , Krstenansky J. , Lee S. , McCarley D. , Mulkins M. , Wong B. , Pease J. , Mizoue L. , Mirzadegan T. , Polsky I. , Thompson K. , Handel T.M. , Jarnagin K.
Biochemistry38:13013-13025(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IMPORTANCE OF TYR-36; ARG-47; LYS-58; LYS-61 AND LYS-72 FOR RECEPTOR BINDING;MUTAGENESIS
25.

"Identification of the glycosaminoglycan binding site of the CC chemokine, MCP-1: implications for structure and function in vivo."
Lau E.K. , Paavola C.D. , Johnson Z. , Gaudry J.-P. , Geretti E. , Borlat F. , Kungl A.J. , Proudfoot A.E. , Handel T.M.
J. Biol. Chem.279:22294-22305(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GAG BINDING SITES ARG-41; LYS-42 AND ARG-47;MUTAGENESIS
26.

"Fat-specific protein 27 modulates nuclear factor of activated T cells 5 and the cellular response to stress."
Ueno M. , Shen W.J. , Patel S. , Greenberg A.S. , Azhar S. , Kraemer F.B.
J. Lipid Res.54:734-743(2013) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INDUCTION BY OSMOTIC STRESS
27.

"Modeling the three-dimensional structure of the monocyte chemo-attractant and activating protein MCAF/MCP-1 on the basis of the solution structure of interleukin-8."
Gronenborn A.M. , Clore G.M.
Protein Eng.4:263-269(1991) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: 3D-STRUCTURE MODELING
28.

"Heteronuclear (1H, 13C, 15N) NMR assignments and solution structure of the monocyte chemoattractant protein-1 (MCP-1) dimer."
Handel T.M. , Domaille P.J.
Biochemistry35:6569-6584(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: STRUCTURE BY NMR
29.

"The structure of MCP-1 in two crystal forms provides a rare example of variable quaternary interactions."
Lubkowski J. , Bujacz G. , Domaille P.J. , Handel T.M. , Wlodawer A.
Nat. Struct. Biol.4:64-69(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS)
30.

"A functional promoter polymorphism in monocyte chemoattractant protein-1 is associated with increased susceptibility to pulmonary tuberculosis."
Flores-Villanueva P.O. , Ruiz-Morales J.A. , Song C.-H. , Flores L.M. , Jo E.-K. , Montano M. , Barnes P.F. , Selman M. , Granados J.
J. Exp. Med.202:1649-1658(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INVOLVEMENT IN MYCOBACTERIUM TUBERCULOSIS SUSCEPTIBILITY

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